Molecular identification of Ralstonia pseudosolanacearum causing bacterial wilt in tomatoes from Da Nang by using Colony PCR

Ralstonia solanacearum species complex (RSSC) is the main pathogen causing bacterial wilt disease in tomatoes. This study applied colony polymerase chain reaction (PCR) technique to rapidly screen and select RSSC strains from isolated bacteria of diseased method directly used colonies on Petri plate as templates amplify with RSSC’s specific multiplex primers. The results showed that Vietnamese isolates were identified R. pseudosolanacearum, phylotype I. Phylogenetic analysis 16S-23S rDNA sequencing also confirmed these results. Therefore, this first report recognize pseudosolanacearum I cause tomatoes Danang.